Multiple Structural Change Algorithm for analysis of cell length profiles in roots

Based on Pacheco-Escobedo MA, Ivanov VB, Ransom-Rodríguez I, Arriaga-Mejía G, Ávila H, Baklanov IA, Pimentel A, Corkidi G, Doerner P, Dubrovsky JG, Álvarez-Buylla ER, Garay-Arroyo A. 2016. Longitudinal zonation pattern in Arabidopsis root tip defined by a multiple structural change algorithm. Annals of Botany 118: 763–776.


This web site performs an analysis of cell length profile using a Multiple Structural Change algorithm for the determination of the domains and zones and boundaries between them in the Arabidopsis thaliana root. The one breakpoint analysis estimates the position of the boundary between the proliferation domain (PD) and the transition domain (TD) of the root apical meristem. It is recommended to use for cell length profiles that include measurements of cell lengths starting from the quiescent centre (QC) of the root apical meristem, till the first few cells of the elongation zone (EZ). The two breakpoints analysis estimates the position of two boundaries: one between the proliferation domain (PD) and the transition domain (TD) of the root apical meristem and another one between the root apical meristem and the EZ. It is recommended to use for cell length profiles that include measurements of cell lengths from the QC to the beginning of the differentiation zone (DZ) It is allowed to include the first few cells of the DZ.

The Multiple Structural Change algorithm was successfully applied to a number of genotypes of Arabidopsis thaliana roots. Potentially it can be applied to other plant species. However, preliminary experiments are required.

Introduce your cell length profile in the following form: 5.3, 4.5, 6.3, ..., 98.5



Cell lengths:



Note 1: Measurement units can be different and depend of the data collected.

Note 2: The above example is for the two breakpoints analysis.